Supercritical angle fluorescence for enhanced axial sectioning in STED microscopy
نویسندگان
چکیده
منابع مشابه
Nanometer axial resolution by three-dimensional supercritical angle fluorescence microscopy.
We report a noninvasive fluorescence microscopy method and demonstrate nanometer resolution along the optical axis. The technique is based on the influence of the microscope slide on the angular intensity distribution of fluorescence. Axial positions are determined by measuring the proportion of light emitted below the critical angle of total internal reflection, which behaves in a classical wa...
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We present an alternative full field imaging technique based on Supercritical Angle Fluorescence (SAF). When fluorescent emitters are placed in the vicinity of the glass slide, their near-field components become propagative at supercritical angles. This supercritical emission decays sharply the fluorophore/surface distance d with a characteristic decay length of about 100 nm. Selecting the supe...
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We study the point-spread and optical transfer-function (OTF) of a stimulated emission depletion (STED)-4Pi fluorescence microscope that provides diffraction unlimited resolution along the optic axis. Our calculations take into account the orientation of the linear transition dipole moment of the fluorescent molecules with respect to that of the focal field. We demonstrate a subdiffraction axia...
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We present a new family of pupil masks to control the axial component of the intensity distribution in the focal region of tightly focused light fields. The filters, which consist of a circular clear pupil with a single shaded ring, allow to control the width of the central lobe of the axial spot together with the residual sidelobes energy. The filters can be applied to improve the optical sect...
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ژورنال
عنوان ژورنال: Methods
سال: 2020
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2019.03.027